Table 2.

Influence of lactate on metabolism of 18:2n-6^a by different bacterial strains during 24 h of incubation (Exp. 2)

Strainb	Control		Lactate		SEM	P-value
	% metabolizedc	Main products formed (% of total intermediates and remaining 18:2n-6)d,e	% metabolizedc	Main products formed (% of total intermediates and remaining 18:2n-6)d,e
Bifidobacterium adolescentis RU 424	76.75	10-OH ∆12–18:1 (62%)	82.22	10-OH ∆12–18:1 (67%)	2.859	0.234
Bifidobacterium pseudolongum RU224	23.06	10-OH ∆12–18:1 (15%)	38.41	10-OH ∆12–18:1 (18%)	3.716	0.033
Butyrivibrio fibrisolvens D1	98.00	t11 18:1 (89%)	97.90	t11 18:1 (90%)	0.217	0.765
Butyrivibrio proteoclasticus P18	98.35	18:0 (69%)	98.54	18:0 (76%)	0.248	0.295
		t11 18:1 (22%)		t11 18:1 (17%)
Cutibacterium acnes DSM 1897	88.41	t10,c12 CLA (75%)	60.79	t10,c12 CLA (39%)	20.522	0.395
		10-OH 12–18:1 (7%)		10-OH 12–18:1 (5%)
Streptococcus equinus Pearl 11	83.96	13-OH ∆9–18:1 (69%)	80.92	13-OH ∆9–18:1 (64%)	1.493	0.223
Streptococcus gallolyticus DSM 16831	89.75	13-OH ∆9–18:1 (47%)	92.77	∆9,14–18:2 (56%)	1.877	0.319
		∆9,14–18:2 (32%)		13-OH ∆9–18:1 (27%)
Megasphaera elsdenii 2602A	81.82	13-OH ∆9–18:1 (63%)	79.13	13-OH ∆9–18:1 (50%)	2.287	0.452
		∆9,14–18:2 (5%)		∆9,14–18:2 (10%)
				c9, t13, t14 18:1 (7%)
Megasphaera elsdenii 5052B	88.01	13-OH ∆9–18:1 (42%)	74.35	13-OH ∆9–18:1 (34%)	1.786	0.006
		10-OH ∆12–18:1 (24%)		10-OH ∆12–18:1 (16%)
				c9, t13, t14 18:1 (5%)

^a The initial amount of 18:2n-6 was 40 μg/mL

^b Only the strains which metabolized 18:2n-6 are shown

^c % metabolized, proportion of the initial 18:2n-6 which was converted after 24 h of incubation

^d Only the intermediates representing ≥5% of the sum of total intermediates and remaining initial 18:2n-6 after 24 h of incubation are presented in this table

^ec, cis; t, trans; CLA, conjugated linoleic acid. For each of the formed intermediates, the proportion of the respective intermediate on the sum of total produced intermediates and remainder of 18:2n-6 after 24 h was calculated