Table 3.

Average proportions^a (% of total intermediates and 18:2n-6) of 18:2n-6 and its biohydrogenation intermediates over a 24 h incubation period under different growth conditions^b with mono-cultures of Butyrivibrio fibrisolvens D1 or Propionibacterium acnes DSM 1897 (Exp. 3)

	B. fibrisolvens			C. acnes			SEM	P-value
	Control	Low pH	DHA	Control	Low pH	DHA		Bacterium	Growth medium	Bacterium × growth medium
18:2n-6	20.72β*	73.86α*	71.49α*	77.00β	86.88α	95.21α	3.918	<  0.001	<  0.001	<  0.001
cis-9, trans-11 CLAc	8.01β	25.49α*	27.24α*	0.54	0.41	0.54	3.273	<  0.001	0.013	0.011
trans-11 18:1	71.10α*	0.55β	1.08β	0.64	4.46	0.33	1.959	<  0.001	<  0.001	<  0.001
trans-10, cis-12 CLAc	<  0.01*	<  0.01*	0.40	21.81α	8.25β	3.76β	2.073	<  0.001	0.001	<  0.001

^a The average proportions of FA over the 24 h incubation period were computed as the area under the curve divided by the total duration of incubation (24 h), using the individual measured proportions for each FA at the different sampling times

^b Low pH, control medium with pH adjusted to 5.5; DHA (docosahexaenoic acid), control medium containing 40 μg/mL of 22:6n-3; all growth media contained 40 μg/mL of 18:2n-6

^c CLA, conjugated linoleic acid

^{α, β} Means differ (P <  0.05) between growth media within the same bacterial species

* Means differ (P <  0.05) between B. fibrisolvens and C. acnes within the same growth medium