Table 4.
Average proportionsa (% of total intermediates and 18:2n-6) of 18:2n-6b and its biohydrogenation intermediates over a 24 h incubation period with different ratios of Butyrivibrio fibrisolvens D1 to Propionibacterium acnes DSM 1897 in the inoculum under control growth conditions (Exp. 4)
| B. fibrisolvens (%)/C. acnes (%) | SEM | P-value | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 100/0 | 50/50 | 10/90 | 2/98 | 0.4/99.6 | 0/100 | Linear | Quadratic | ||
| 18:2n-6 | 12.35 | 19.59 | 34.90 | 49.57 | 53.30 | 66.65 | 12.928 | < 0.001 | < 0.001 |
| cis-9, trans-11 CLAc | 19.22 | 22.44 | 10.57 | 8.89 | 4.15 | 0.02 | 7.797 | 0.013 | 0.090 |
| trans-11 18:1 | 67.98 | 51.72 | 34.59 | 14.22 | 15.62 | 0.41 | 14.947 | < 0.001 | 0.035 |
| trans-10, cis-12 CLAc | < 0.01 | 3.03 | 11.55 | 20.08 | 19.13 | 32.92 | 3.123 | < 0.001 | 0.007 |
| trans-10 18:1 | 0.45 | 3.22 | 8.39 | 7.24 | 7.80 | < 0.01 | 4.090 | 0.014 | 0.471 |
| trans-10/trans-11d | – | 0.08 | 0.48 | 3.00 | 4.04 | – | 4.436 | < 0.001 | < 0.001 |
a The average proportions of FA over the 24 h incubation period were computed as the area under the curve divided by the total duration of incubation (24 h), using the individual measured proportions for each FA at the different sampling times
b The initial amount of 18:2n-6 was 40 μg/mL
c CLA, conjugated linoleic acid
d Ratio of trans-10 intermediates (trans-10, cis-12 CLA + trans-10 18:1) to trans-11 intermediates (cis-9, trans-11 CLA + trans-11 18:1)
-: The ratio is not relevant for mono-cultures as trans-11 and trans-10 are produced exclusively with mono-cultures of B. fibrisolvens and C. acnes, respectively