Table 7.

Overview of the different pure culture experiments conducted

	Bacteriuma (inoculum size, v/v)	Ratio of B. fibrisolvens (%)/C. acnes (%) in the inoculumb	Growth mediumc	Fatty acid substrated	Incubation period (h)
Exp. 1	28 strains individually (Table 1) (5%)	NA	Control	18:3n-3	24
				18:2n-6	24
				trans-11 18:1	24
Exp. 2	28 strains individually (Table 1) (5%)	NA	Lactate	18:2n-6	24
Exp. 3	B. fibrisolvens (5%)	100/0	Control	18:2n-6	0, 2, 4, 8, and 24
			Low pH	18:2n-6	0, 2, 4, 8, and 24
			DHA	18:2n-6	0, 2, 4, 8, and 24
	C. acnes (5%)	0/100	Control	18:2n-6	0, 2, 4, 8, and 24
			Low pH	18:2n-6	0, 2, 4, 8, and 24
			DHA	18:2n-6	0, 2, 4, 8, and 24
Exp. 4	B. fibrisolvens (10%)	100/0	Control	18:2n-6	0, 4, 8, and 24
			Low pH	18:2n-6	24
			DHA	18:2n-6	24
	B. fibrisolvens (5%) and C. acnes (5%)	50/50	Control	18:2n-6	0, 4, 8, and 24
			Low pH	18:2n-6	24
			DHA	18:2n-6	24
	B. fibrisolvens (1%) and C. acnes (9%)	10/90	Control	18:2n-6	0, 4, 8, and 24
			Low pH	18:2n-6	24
			DHA	18:2n-6	24
	B. fibrisolvens (0.2%) and C. acnes (9.8%)	2/98	Control	18:2n-6	0, 4, 8, and 24
			Low pH	18:2n-6	24
			DHA	18:2n-6	24
	B. fibrisolvens (0.04%) and C. acnes (9.96%)	0.4/99.6	Control	18:2n-6	0, 4, 8, and 24
			Low pH	18:2n-6	24
			DHA	18:2n-6	24
	C. acnes (10%)	0/100	Control	18:2n-6	0, 4, 8, and 24
			Low pH	18:2n-6	24
			DHA	18:2n-6	24

^a Fresh cultures, grown in modified control medium (30% rumen fluid instead of 20%, v/v) for 12 to 36 h, depending on the growth rate of the strain (OD₆₀₀ = 1.26 ± 0.41; mean ± SD), were used as inoculum

^b NA, not applicable

^c Lactate, control medium supplemented with 200 mM Na-lactate; low pH, control medium with pH adjusted to 5.5; DHA (docosahexaenoic acid), control medium containing 40 μg/mL of 22:6n-3

^d The initial amount of fatty acid was 40 μg/mL