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. 2020 Jul 6;20:291. doi: 10.1186/s12935-020-01397-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — RNF181 promotes cell proliferation and migration in triple negative breast cancer cells. a, b Depletion of RNF181 inhibits the proliferation of triple negative breast cancer cells. BT549 and MDA-MA-231 were transfected with siControl or siRNF181. There were two different siRNA be used. After 24 h, the assay was CCK8 used to determine the cellar metabolic activity at indicated time points after infection. Experiments were done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for cell growth comparison. c–f Wound-healing assay of BT549 and MDA-MB-231 cells were transfected with siControl or siRNF181. Quantification of wound closure at the indicated time points. Data are presented as ± SD. **, P < 0.01, ***, P < 0.001. g, h RNF181 depletion inhibits TNBC cell migration in BT549 and MDA-MB-231 cells. Two independent siRNA were used in the study. Transwell was used to check the migration capacity. The cell number was counted and data are presented as ± SD. **, P < 0.01, ***, P < 0.001