Fig. 3.

RNF181 promotes Hippo signaling in triple negative breast cancer cells. a RNF181 consumption decreased YAP protein levels in BT549 cells. BT549 cells were transfected with siControl or siRNF181. After 48 h, cells were harvested for western blot analysis. RNF181 and YAP protein levels were determined by Western blot. Actin was used as internal control. b RNF181 consumption decreased YAP protein levels in MDAMB231 cells. MDAMB231 cells were transfected with siControl or siRNF181. After 48 h, cells were harvested for western blot analysis. RNF181 and YAP protein levels were determined by Western blot. Actin was used as internal control. c RNF181 depletion decreased TEAD Luciferase activity in BT549 cells. BT549 cells were transfected with siControl or siRNF181. After 24 h, cells were transfected with TEAD luciferase reporter plasmids. After 24 h, cells harvested for luciferase activity analysis. d RNF181 depletion decreased TEAD Luciferase activity in MDAMB231 cells. MDAMB231 cells were transfected with siControl or siRNF181. After 24 h, cells were transfected with TEAD luciferase reporter plasmids. After 24 h, cells harvested for luciferase activity analysis. e RNF181 consumption decreased YAP target gene expression in BT549 cells. BT549 cells were transfected with siControl or siRNF181. After 48 h, total RNA was extracted for gene expression analysis. *P < 0.05; ** P < 0.01; ***P < 0.001 for target gene expression comparison. f RNF181 consumption decreased YAP target gene expression in MDAMB231 cells MDAMB231 cells were transfected with siControl or siRNF181. After 48 h, total RNA was extracted for gene expression analysis. *P < 0.05; ** P < 0.01; ***P < 0.001 for target gene expression comparison