Fig. 4.

RNF181 promotes cancer cell migration and invasion through Hippo/YAP signaling. a RNF181 consumption decreased YAP protein level, which effect could be reversed by YAP over-expressed. BT549 cells were transfected with siControl or siRNF181. After 24 h, cells were transfected with Myc-YAP or Myc. After 48 h, cells were harvested for western blot analysis. RNF181 and YAP protein levels were determined by Western blot. Actin was used as internal control. b, c Wound-healing assay indicated that RNF181 consumption decreased BT549 cell migration capacity, which effect could be reversed by YAP over-expressed. BT549 cells were transfected with siControl or siRNF181. After 24 h, cells were transfected with Myc-YAP or Myc. Quantification of wound closure at the indicated time points. Data are presented as ± SD. **, P < 0.01, ***, P < 0.001 (student’s t-test). d, e Wound-healing assay indicated that RNF181 consumption decreased MDAMB231 cell migration capacity, which effect could be reversed by YAP over-expressed. MDAMB231 cells were transfected with siControl or siRNF181. After 24 h, cells were transfected with Myc-YAP or Myc. Quantification of wound closure at the indicated time points. Data are presented as ± SD. **, P < 0.01, ***, P < 0.001 (student’s t-test). f, g RNF181 consumption decreased TNBC cell invasion capacity, which effect could be reversed by YAP over-expressed. BT549 cells were transfected with siControl or siRNF187. After 24 h, cells were transfected with Myc-YAP or Myc. After another 24 h, cancer cells were seeded into the chamber for trans-well assay. The cell number was counted and Data are presented as ± SD. **, P < 0.01, ***, P < 0.001 (student’s t-test)