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. 2020 Jul 6;17:202. doi: 10.1186/s12974-020-01884-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — LRCH1 expression is reduced in post-SCI microglia. a The gating and sorting of spinal cord microglia using flow cytometry. Granulocyte^- cells were first gated from the whole enriched leukocytes, and CD45^lowCD11b⁺ microglia were then recognized and sorted. d2~d7, day 2 to day 7 after SCI. b The purity of sorted microglia. Sorted microglia were re-tested for the expression of CD45 and CD11b. c LRCH1 mRNA levels in sorted microglia at indicated time points after SCI. Sh, sham control. n = 5 in 3 independent experiments. d LRCH1 protein levels in sorted microglia. The data represents two independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001