Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 6;20:289. doi: 10.1186/s12935-020-01390-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — The expression patterns of circRNA CDR1as, miR-641 and HOXA9 in human NSCLC cell lines (A549, H1299 and Calu6) and their paired descendent cisplatin-resistant sub-lines (A549/DDP, H1299/DDP and Calu6/DDP). The above cells were cultured in standard conditions and subsequently stimulated with high-dose cisplatin for 48 h, cell proliferation was measured by a CCK-8 assay and b trypan blue staining method was employed to detect cell viability. The results indicated that A549/DDP, H1299/DDP and Calu6/DDP were more resistant to high-dose cisplatin treatment compared to the parental DDP-sensitive NSCLC cells. The expression levels of c circRNA CDR1as and e HOXA9 mRNA were upregulated, while d miR-641 was downregulated in DDP-resistant NSCLC cells compared to DDP-sensitive cells, determined by using Real-Time qPCR. f, g Western Blot results showed that HOXA9 protein levels were increased in DDP-resistant NSCLC cells. Each experiment repeated at least 3 times. **P < 0.01