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. 2020 Jul 6;20:289. doi: 10.1186/s12935-020-01390-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — CircRNA CDR1as/miR-641/HOXA9 axis regulated stemness of NSCLC cells in vitro. a The spheroid formation assay results indicated that DDP-resistant NSCLC cells tended to form spheres in contrast with their corresponding parental DDP-sensitive NSCLC cells. b–d The mRNA levels of SOX2, OCT4 and Nanog were upregulated in DDP-resistant NSCLC cells compared to DDP-sensitive NSCLC cells, examined by Real-Time qPCR. e Flow cytometer was used to measure the proportion of CD44⁺CD166⁺ cells in NSCLC cells. f–h Upregulation of circRNA CDR1as promoted stemness associated signatures expression in DDP-sensitive NSCLC cells by regulating miR-641/HOXA9 axis. i–k Knock-down of circRNA CDR1as inhibited stemness properties in DDP-resistant NSCLC cells by targeting miR-641/HOXA9 axis. Each experiment repeated at least 3 times. *P < 0.05, **P < 0.01. “NS” represented no statistical significance