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. 2020 Jul 6;39:128. doi: 10.1186/s13046-020-01638-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — NCSTN promotes HCC cell growth and metastasis in vitro. a The effects of NCSTN knockdown and overexpression were examined by western blotting analysis in HCCLM3 and Hep3B cells. Loading control was assessed by β-actin. b CCK8 assays showed NCSTN depletion inhibited cell growth of HCCLM3 and NCSTN overexpression promoted cell growth of Hep3B. c, d Colony formation assays showed colony numbers in HCC cells with NCSTN depletion or overexpression. e, f The cell cycle assays showed that NCSTN depletion increased the G0/G1 fraction and decreased the S and G2/M fraction in HCCLM3 cells, whereas NCSTN overexpression decreased the G0/G1 fraction and increased the S and G2/M fraction in Hep3B cells. g, h The migration and invasion capacity was determined in the indicated HCC cells. Scale bar, 100 μm. i, j Wound healing assays showed the migration capacity of indicated HCC cells. Scale bar, 100 μm. HCC, hepatocellular carcinoma; CCK8, cell counting kit-8. *p < 0.05, **p < 0.01, ***p < 0.001