Figure 3.

Knockdown of miR-106a-5p inhibits ox-LDL-induced ROS in HUVECs. After transfection with a miR-106a-5p inhibitor or inhibitor NC, HUVECs were exposed to ox-LDL (80 µg/ml) for 48 h. (A) ROS levels were detected using a ROS detection kit. (B) Relative fluorescence intensity is shown in the bar graphs. (C) p22phox protein level was detected using western blotting; β-actin was used as an internal control. *P<0.05, **P<0.01 vs. blank group; ^##P<0.01 vs. inhibitor NC. ROS, reactive oxygen species; ox-LDL, oxidized low-density lipoproteins; miR, microRNA; NC, negative control; HUVECs, human umbilical vein endothelial cells.