Figure 6.

Effects of HR and MAPK inhibitor U0126 on the expression levels of TLR4, NF-κB, IκB and p-MAPK-1 in HSCs. After treating hepatic stellate cells with or without MAPK inhibitor U0126 (10 µM), cells were placed in a 94% N₂, 5% CO₂ and 1% O₂ humidified culture incubator for 6 h, followed by reoxygenation with 5% CO₂ and 95% air for 12 h. Protein expression was determined by western blot analysis. (A) Representative protein bands. Protein expression levels of (B) TLR4, (C) NF-κB, (D) IκB and (E) p-MAPK-1. Data are presented as the mean ± SEM, n=6 in each group. **P<0.01 vs. control; ^{#P<0.05, ##}P<0.01 vs. HR. Con, control; p, phosphorylated; IκB, inhibitor of NF-κB; TLR4, Toll-like receptor 4; MAPK, mitogen-activated protein kinase; HR, hypoxia/reoxygenation.