Figure 4.

FGFR2 is a direct target of miR-217-5p. (A) Potential targets of miR-217-5p were identified in the 3′-UTR of FGFR2 through TargetScan. (B) Skeletal muscle stem cells transfected with the miR-217-5p mimic, NC-antagomir, NC-mimics or miR-192-5p antagomir in the presence of WT or MUT FGFR2 3′-UTR, and the binding was detected by measuring luciferase activity. (C-E) Reverse transcription-quantitative PCR and western blotting were performed to identify the (C) mRNA and (D and E) protein expression levels of FGFR2 in transfected cells. The level of FGFR2 was normalized to that of GAPDH. *P<0.05. miR, microRNA; NC, control group; UTR, untranslated region; FGFR2, fibroblast growth factor receptor 2; WT, wild-type; MUT, mutant.