Figure 4.

IL-6 and TNF-α expression in the four treatment groups and the effect of TIM-3 overexpression on the NF-κB signaling pathway in PDGF-BB-stimulated HASMCs. Protein expression levels of (A) IL-6, (B) TNF-α, (C) TIM-3, p-p65 NF-κB and p65 NF-κB were detected by western blotting. *P<0.05, **P<0.01. IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; TIM-3, T-cell immunoglobulin and mucin domain 3; PDGF-BB, platelet-derived growth factor-BB; HASMCs, human artery vascular smooth muscle cells; p, phosphorylated; LV, lentivirus.