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. Author manuscript; available in PMC: 2021 Mar 17.
Published in final edited form as: Anal Chem. 2020 Feb 28;92(6):4459–4467. doi: 10.1021/acs.analchem.9b05481

Figure 1. Tandem-TIMS/MS retains intact, native-like avidin tetramers.

Figure 1.

(A) Tetramers with charge states 17+ to 19+ predominate in the native mass spectrum recorded for avidin by tandem-TIMS/MS. (B) Cross sections obtained for avidin tetramers by tandem-TIMS (black circles) agree with cross sections obtained on a drift-tube (red squares) and the x-ray structures (shaded). (C) Mass spectrum obtained for avidin tetramers 18+ and 19+ with calculated m/z for non-glycosylated and glycosylated tetramers for charge state 18+ (red arrows). (D) Broad tetramer peaks are observed in the ion mobility spectra, here shown for charge state 18+.