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. Author manuscript; available in PMC: 2021 Mar 17.
Published in final edited form as: Anal Chem. 2020 Feb 28;92(6):4459–4467. doi: 10.1021/acs.analchem.9b05481

Figure 2. Tandem-ion mobility experiments for avidin tetramer 18+.

Figure 2.

(A) A broad peak is observed for avidin tetramers 18+ upon mobility analysis in TIMS-1. (B) Four mobility windows (“slices”) within tetramer 18+ are selected in the interface of tandem-TIMS and transmitted through TIMS-2. The mobility-selected regions reconstruct the shape of the full tetramer 18+ peak shown in (A). (C) Nine mobility “slices” are selected in the interface and mobility-analyzed in TIMS-2. The overlay of mobility-selected peaks reconstructs the full tetramer 18+ peak. For clarity, the abundances of the mobility-selected traces are scaled by a constant factor of 1.56.