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. Author manuscript; available in PMC: 2021 Mar 17.
Published in final edited form as: Anal Chem. 2020 Feb 28;92(6):4459–4467. doi: 10.1021/acs.analchem.9b05481

Figure 4. Collision-induced dissociation (CID) of native-like avidin tetramers in the interface of tandem-TIMS.

Figure 4.

(A) At an activation voltage of 140 V, avidin dissociates mainly into extended monomers and compact trimers indicating a “typical” CID mechanism. (B) At an activation voltage of 260 V, compact monomers and dimers emerge, which indicates the presence of an “atypical” CID mechanism at higher activation voltages. (C) Cross section distributions for avidin trimers 10+ generated at 120 V (black), 160 V (blue), and 240 V (red) reveal that the trimers unfold at higher activation voltages. (D) The breakdown graph reveals presence of an “atypical” CID mechanism at activation voltages above ~150 V.