Figure 3.

TBMS1 induces the apoptosis of glioma cells by blocking Bcl-2. U251 and HA cells were incubated with 20, 30 or 40 µg/ml of TBMS1 for 24 h. The cells were stained using (A) Hoechst 33342 staining and (B) Annexin V-FITC/PI double staining. A statistical analysis was performed for apoptosis. (C) The expression levels and quantitative values of Bad, Bax, PARP, caspase-3, caspase-9, AIF and Bcl-2 were monitored via western blot analysis. *P<0.05, **P<0.01 and ***P<0.001 vs. TBMS1 (0 µg/ml). TBMS1, tubeimoside-1; FITC, fluorescein 5-isothiocyanate; PI, propidium iodide; PARP, poly-ADP ribose polymerase; AIF, apoptosis-inducing factor.