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. 2020 Jun 2;22(2):1119–1128. doi: 10.3892/mmr.2020.11200

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression level of IHH in LF and OLF tissues. (A) Reverse transcription-quantitative PCR results showed the mRNA expression levels of IHH between the LF group and OLF group. (B) Representative images of immunohistochemistry staining from each group. Magnification, ×400. The red arrows represent the typical cells, which were magnified and shown in the upper right-hand corner (magnification, ×1,600). (C) Immunohistochemistry results of the protein expression levels of IHH in the LF group and OLF group. N=8. **P<0.01 vs. LF samples. IHH, Indian hedgehog; OLF, ossification of ligamentum flavum; LF, ligamentum flavum.