Figure 3.

Expression level of osteogenic genes and IHH signaling genes in LF and OLF cells after cyclic stretch. (A) Reverse transcription-quantitative PCR results showed that in LF cells, cyclic stretch increased the ALP expression level, while other gene expression levels did not change. (B) Relative mRNA expression levels of RUNX2, Osterix, ALP, OCN, IHH and SMO significantly increased after the application of cyclic stretch in OLF cells. *P<0.05, **P<0.01 vs. 0 h. Data are presented as the mean ± SD. N=3. OLF, ossification of ligamentum flavum; LF, ligamentum flavum; RUNX2, Runt-related transcription factor 2; ALP, alkaline phosphatase; OCN, osteocalcin; IHH, Indian hedgehog.