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. 2020 Jun 2;22(2):1119–1128. doi: 10.3892/mmr.2020.11200

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of cyclic stretch on osteogenic genes and ALP activity. Expression levels of (A) RUNX2, (B) Osterix, (C) ALP and (D) OCN in OLF cells were detected after cyclic stretch treatment for 0, 6, 12 and 24 h. Protein expression levels of (E) RUNX2, (F) Osterix, (G) ALP and (H) OCN after cyclic stretch. (I) Representative images of ALP staining. Magnification, ×400. (J) Cyclic stretch increased ALP activity in OLF cells. *P<0.05, **P<0.01, ***P<0.001. Data are presented as the mean ± SD. N=3. OLF, ossification of ligamentum flavum; RUNX2, Runt-related transcription factor 2; ALP, alkaline phosphatase; OCN, osteocalcin.