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. 2020 Jun 2;22(2):1119–1128. doi: 10.3892/mmr.2020.11200

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effect of the Indian hedgehog signaling pathway inhibitor Cpn on the expression levels of RUNX2, Osterix, ALP and OCN in OLF cells. mRNA expression levels of (A) RUNX2, (B) Osterix, (C) ALP and (D) OCN. Protein expression levels of (E) RUNX2, (F) Osterix, (G) ALP (H) and OCN were significantly decreased in the stretch + Cpn group compared with cyclic stretch alone. (I) Representative images of ALP staining. Magnification, ×400. (J) ALP activity was significantly reduced in the Cpn + stretch group compared with cyclic stretch alone. *P<0.05, **P<0.01, ***P<0.001. Data are presented as the mean ± SD. N=3. OLF, ossification of ligamentum flavum; RUNX2, Runt-related transcription factor 2; ALP, alkaline phosphatase; OCN, osteocalcin; Cpn, cyclopamine.