Table I.

Primers used for reverse transcription-quantitative PCR.

Target	Primer sequence	Genbank accession no.
GAPDH	F: 5′-AGGTCGGTGTGAACGGATTTG-3′	NM_008084
	R: 5′-TGTAGACCATGTAGTTGAGGTCA-3′
RUNX2	F: 5′-ATGCTTCATTCGCCTCACAAA-3′	NM_001146038
	R: 5′-GCACTCACTGACTCGGTTGG-3′
Osterix	F: 5′-ATGGCGTCCTCTCTGCTTG-3′	AF477981.1
	R: 5′-TGAAAGGTCAGCGTATGGCTT-3′
ALP	F: 5′-CCAACTCTTTTGTGCCAGAGA-3′	NM_007431
	R: 5′-GGCTACATTGGTGTTGAGCTTTT-3′
OCN	F: 5′-CTGACCTCACAGATCCCAAGC-3′	X04142
	R: 5′-TGGTCTGATAGCTCGTCACAAG-3′
IHH	F: 5′-CGGCTTTGACTGGGTGTATT-3′	KR710697.1
	R: 5′-AAAATGAGCACATCGCTGAA-3′
SMO	F: 5′-CTGTCCTGCGTCATCATCTTT-3′	NM_005631.5
	R: 5′-CCACAGCAAGGATTGCCAC-3′
GLI1	F: 5′-AAGCGTGAGCCTGAATCTGT-3′	BC013000.2
	R: 5′-CAGCATGTACTGGGCTTTGA-3′
GLI2	F: 5′-CGACACCAGGAAGGAAGGTA-3′	BC172434.1
	R: 5′-TGCACAGAACGGAGGTAGTG-3′
GLI3	F: 5′-CTTTGCAAGCCAGGAGAAAC-3′	BC113616.1
	R: 5′-TTGTTGGACTGTGTGCCATT-3′

F, forward; R, reverse; ALP, alkaline phosphatase; RUNX2, Runt-related transcription factor 2; OCN, osteocalcin; IHH, Indian hedgehog; SMO, Smoothened; GLI, GLI family zinc finger.