Skip to main content
. 2020 May 27;22(2):1382–1390. doi: 10.3892/mmr.2020.11188

Figure 1.

Figure 1.

Expression of USP51 and ZEB1 is significantly increased in A549 or A549/DDP cells. Total RNA and protein were extracted from A549, A549/DDP and 16HBE cells. (A) After treatment with gradient concentrations of DDP (0, 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 µmol/l), cell proliferation was assessed to determine drug resistance in the indicated cells (A549 and A549/DDP) to DDP. The mRNA expression of (B) USP51 and (C) ZEB1 was detected by reverse transcription-quantitative PCR. (D) Protein expression levels of USP51 and ZEB1 were detected by western blotting. **P<0.01 and ***P<0.001 vs. 16HBE, and ##P<0.01 and ###P<0.001 vs. A549. A549, lung cancer cells; A549/DDP, DDP-resistant A549 cells; 16HBE, normal lung cells. USP, ubiquitin-specific protease; ZEB1, zinc-finger E-box binding homeobox 1.