Figure 2.

Knockdown or overexpression of USP51 in A549 or A549/DDP cells by lentivirus infection. A549 cells were infected with USP51/vector while A549/DDP cells were infected with shUSP51/shNC. (A and B) USP51 mRNA expression (upper) and protein levels (lower) in (A) A549 or (B) A549/DDP cells were determined by reverse transcription-quantitative PCR and western blotting, respectively. ***P<0.001 vs. vector or shNC. A549, lung cancer cells; A549/DDP, DDP-resistant A549 cells. USP, ubiquitin-specific protease; sh, short hairpin RNA; NC, negative control.