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. 2020 May 22;22(2):870–878. doi: 10.3892/mmr.2020.11181

Figure 3.

Figure 3.

Adiponectin inhibited GCIR-induced apoptosis in brain tissues. (A) TUNEL-positive cells were detected by HRP-conjugated probes with DAB staining in brain tissues of different groups; I: Sham group, II: PCAS group, III: APN group, arrows indicate apoptotic cells. (B) Expression of cleaved casepase-3 in the cortex of different groups mice at 3 h of reperfusion; I: Sham group, II: PCAS group, III: APN group, arrows indicated the apoptotic cells. Nuclei were stained by DAPI (blue). Apoptotic protein cleaved casepase-3, anti-apoptotic protein Bcl-2 and activation of AMPK were measured by western blotting in brain tissues of different groups. (C) The grayscale values of (D) casepase-3, (E) Bcl-2 and (F) activation of AMPK in different groups. Data are presented as means ± standard errors of the mean (n=5 per group). *P<0.05 vs. Sham group. #P<0.05 vs. PCAS group. GCIR, global cerebral ischemia-reperfusion; HRP, horseradish peroxidase; DAB, 3,3′-diaminobenzidine; DAPI, 4′,6-diamidino-2-phenylindole; AMPK, AMP-activated protein kinase; APN, adiponectin; PCAS, post cardiac arrest syndrome.