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. 2020 Jun 15;22(2):1179–1186. doi: 10.3892/mmr.2020.11228

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Copyright: © Tao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Overexpression of Septin4 promotes apoptosis in hFOB 1.19 cells treated with a high melatonin concentration. (A) hFOB cells were transfected with NC plasmid or Flag-Septin4 plasmid for 48 h and treated with 0, 2, 4 and 6 mmol/l of melatonin for 48 h. Cell viability was assessed via the MTT assay. (B) hFOB cells were transfected with NC plasmid or Flag-Septin4 plasmid for 48 h and treated with 0 or 4 mmol/l of melatonin for 48 h. Flow cytometry was performed to analysis cell apoptosis (Q4 region). *P<0.05, **P<0.01, ***P<0.001, vs. NC group. GRP, glucose-regulated protein; NC, negative control; Con, control; PI, propidium iodide.

Figure 2. — Overexpression of Septin4 promotes apoptosis in hFOB 1.19 cells treated with a high melatonin concentration. (A) hFOB cells were transfected with NC plasmid or Flag-Septin4 plasmid for 48 h and treated with 0, 2, 4 and 6 mmol/l of melatonin for 48 h. Cell viability was assessed via the MTT assay. (B) hFOB cells were transfected with NC plasmid or Flag-Septin4 plasmid for 48 h and treated with 0 or 4 mmol/l of melatonin for 48 h. Flow cytometry was performed to analysis cell apoptosis (Q4 region). *P<0.05, **P<0.01, ***P<0.001, vs. NC group. GRP, glucose-regulated protein; NC, negative control; Con, control; PI, propidium iodide.