Figure 2.

Effects of troxerutin on PI3K/AKT/HIF-1α signaling pathway under OGD/R condition in H9C2 cells. H9C2 cells were pretreated with troxerutin (10 µM) and then co-treated with or without OGD (6 h)/R (18 h). (A) The expression levels of PI3K, p-AKT, AKT and HIF-1α were determined using western blot analysis. Quantitative analysis of (B) PI3K, (C) p-AKT/AKT ratio and (D) HIF-1α expression in H9C2 cells. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 vs. -OGD/R-troxerutin; ^#P<0.05, ^##P<0.01 vs. +OGD/R-troxerutin. OGD/R, oxygen-glucose deprivation and reoxygenation; HIF-1α, hypoxia-inducible factor-1α; p-, phosphorylated.