Skip to main content
. 2020 Jun 3;22(2):1351–1361. doi: 10.3892/mmr.2020.11207

Figure 4.

Figure 4.

Effects of HIF-1α-siRNA or NS-siRNA on TX-induced inhibition of cytotoxicity and apoptosis in OGD/R-treatedH9C2 cells. H9C2 cells were transfected with HIF-1α-siRNA or NS-siRNA for 6 h and then treated with TX (10 µM) followed by OGD (6 h)/R (18 h) treatment. (A) The relative level of HIF-1α mRNA was determined using reverse transcription-quantitative PCR and the relative level of mRNA was analyzed using the 2−ΔΔCq method. (B) Cell viability was determined using the MTT assay. (C) LDH activity was determined using a commercial LDH assay kit. (D) The percentage of apoptosis was determined using an Annexin V-FITC/propidium iodide staining kit. (E) Quantification of flow cytometry analysis of H9C2 cell apoptosis. Data are presented as the mean ± SD (n=3). **P<0.01 vs. NS-siRNA + control group; #P<0.05, ##P<0.01 vs. NS-siRNA + OGD/R group; &P<0.05, &&&P<0.001 vs. NS-siRNA +TX + OGD/R group. OGD/R, oxygen-glucose deprivation and reoxygenation; siRNA, small interfering RNA; HIF-1α, hypoxia-inducible factor-1α; NS, non-specific; TX, troxerutin; LDH, lactate dehydrogenase.