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. 2020 May 15;22(2):723–732. doi: 10.3892/mmr.2020.11149

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Expression of proliferation- and invasion-related markers in GBM cells. (A) Correlation between FLVCR1-AS1 and MMP-2/MMP-9 expression in sample data from TCGA GBM database. Following transfection with si-FLVCR1-AS1 or si-NC, the (B) protein and (C) mRNA expression levels of PCNA, MMP-2 and MMP-9 were determined. (D) Immunofluorescence staining revealed a decreased level of MMP-2 in si-FLVCR1-AS1-transfected cells (magnification, ×400). *P<0.05 and **P<0.01 vs. si-NC. Experiment was repeated three times. GBM, glioblastoma; FLVCR1-AS1, Feline Leukemia Virus Subgroup C Cellular Receptor 1 Antisense RNA 1; MMP, matrix metalloproteinase; TCGA, The Cancer Genome Atlas; si-, small interfering; NC, negative control; si, small interfering RNA; TCGA, The Cancer Genome Atlas.