Figure 2.

miR-205 silences MDM4 by binding with its 3′UTR. (A) TargetScan prediction software was used to scan 3′UTR of MDM4 gene to detect the miR-205 combining site. (B) WT and MT MDM4 3′UTR were cloned to the downstream of firefly luciferase in the psiCHECK-2 vector. Then, plasmids with miR-205 mimic/NC were co-transfected into 293T cells. Luciferase activity was analyzed using a Nano-Glo Dual-Luciferase Reporter Assay System. (C) miR-205 expression was determined via reverse transcription-quantitative PCR. **P<0.01 and ***P<0.001. miR, microRNA; MDM4, mouse double minute 4; WT, human wild-type; MT, mutant; NC, mimic negative control; UTR, untranslated region.