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Change in absorbance due to temperature for (A) tryptophan at 280 nm and (B) uracil at 260 nm. The change in absorbance (Δ Absorbance) is calculated by ΔtA = A22 °C − At, where t is the measured temperature in degrees Celsius. The error bars are 1 standard deviation of the mean. The gray area around the line is the 95 % uncertainty calculation for the fit of the line to the data.
