Fig. 5.
Change in absorbance as a function of spectral bandwidth for (A) tryptophan at 280 nm and (B) uracil at 260 nm. Data shown are for 1 mm pathlength and for 0.5 mm pathlength that has been normalized to 1 mm. Here, the change in absorbance (Δ Absorbance) is calculated by ΔBA = A0.8 nm − Abandwidth, where B is the spectral bandwidth in nm. The error bars are 1 standard deviation of the mean. The gray area around the line is the 95 % uncertainty calculation for the fit of the line to the data.