Figure 1.

Effects of PA on human HepG2 cells. (A) PA did not affect cell growth but induced lipid accumulation in HepG2 cells under 10% FBS culture medium. Scale bar, 100 µm. The 3% (w/v) FFA-free BSA solution was prepared in DMEM (vehicle) and was used to prepare PA solution. (B) Effects of PA on human HepG2 CSCs under sphere formation culture conditions. Scale bar, 100 µm. (C) PA significantly increased the number of human HepG2 CSC spheres. Data are presented as the mean ± standard error of the mean; n=3. ***P<0.001. PA, palmitate; CSC, cancer stem cell.