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. 2020 May 18;22(2):1026–1034. doi: 10.3892/mmr.2020.11155

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Copyright: © Fu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Excessive accumulation of autophagosomes does not contribute to SSD-mediated apoptosis. Stable knockdown MDA-MB-231 cells (shCON or shATG5) were exposed to 10 µM SSD for 24 h. (A) Following treatment, the expression levels of ATG5, LC3B-II, p-p38, p38, c-caspase-3 and c-PARP were detected using western blotting. (B) Semi-quantitative analysis of the expression levels in part A, n=3; **P<0.01. (C) Apoptotic cells were determined using flow cytometry. (D) Quantitative analysis of the apoptotic rate form part C. n=3. (E) Cell viability was determined using MTT assays. n=3. All data are presented as the mean ± SD. sh, small hairpin RNA; CON, control; ATG, autophagy-related protein; c-, cleaved; n.s., not significant; p-, phosphorylated; LC3B, microtubule-associated protein 1 light chain 3 β; PARP, poly (ADP-ribose) polymerase; SSD, saikosaponin D; PI, propidium iodide.