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. 2020 Jun 16;22(2):1373–1381. doi: 10.3892/mmr.2020.11242

Figure 1.

Figure 1.

Overexpression or knockdown of RP105 in rat cardiac muscle tissue. (A) Expression levels of EGFP, with the indicated adenovirus gene injection for RP105 overexpression and RP105-siRNA, and DAPI-labeled nuclei density of cardiomyocytes. Scale bar, 50 µm. (B) mRNA expression of RP105 in rat cardiac muscle tissues was detected by reverse transcription-quantitative PCR after transfection with the indicated vector. (C) Protein expression levels of RP105 in rat cardiac muscle tissue. *P<0.05, **P<0.01, ***P<0.001. Ad, adenovirus; RP105, radioprotective 105 kDa protein; siRNA, small interfering RNA; EGFP, enhanced green fluorescent protein; sham, control sham group; I/R, ischemic and reperfusion; Ad-R, Ad-RP105 group; Ad-R-siRNA, Ad-RP105-siRNA group; Ad-G-siRNA, Ad-EGFP-siRNA group.