Overexpression or knockdown of RP105 in rat cardiac muscle tissue. (A) Expression levels of EGFP, with the indicated adenovirus gene injection for RP105 overexpression and RP105-siRNA, and DAPI-labeled nuclei density of cardiomyocytes. Scale bar, 50 µm. (B) mRNA expression of RP105 in rat cardiac muscle tissues was detected by reverse transcription-quantitative PCR after transfection with the indicated vector. (C) Protein expression levels of RP105 in rat cardiac muscle tissue. *P<0.05, **P<0.01, ***P<0.001. Ad, adenovirus; RP105, radioprotective 105 kDa protein; siRNA, small interfering RNA; EGFP, enhanced green fluorescent protein; sham, control sham group; I/R, ischemic and reperfusion; Ad-R, Ad-RP105 group; Ad-R-siRNA, Ad-RP105-siRNA group; Ad-G-siRNA, Ad-EGFP-siRNA group.