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. Author manuscript; available in PMC: 2020 Nov 18.
Published in final edited form as: Nat Struct Mol Biol. 2020 May 18;27(6):570–580. doi: 10.1038/s41594-020-0426-4

Fig. 5: Helical contacts are required for efficient HIV-1 release.

Fig. 5:

Depletion of endogenous VPS28 with RNAi (lanes 2–8) and reconstitution with empty vector (lane 2), WT VPS28 (lanes 3–5), or VPS28 TM mutant (lanes 6–8) in 293T cells. Cells were co-transfected with an HIV-1 mutant with the LYPnXL L domain abrogated and virus release was compared with a non-depleted control (lane 1). Virion production and Gag expression levels were compared by Western blotting with anti-NC and anti-CA antibodies and cellular protein levels were probed with anti-VPS28 and anti-tubulin antibodies. The images shown are representative of three biological replicates of the experiment. Uncropped images are in Supplementary Figure 1.