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. 2020 Jun 30;8:653. doi: 10.3389/fbioe.2020.00653

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Copyright © 2020 Liu, Dong, Qi, Zhang, Huang, Xu and Lian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of fermentation conditions for crocetin production in the engineered strain TL009. (A) Time course of crocetin production with TL009 at 24°C. (B) Growth curves of the engineered yeast strain TL009 at 30°C (black squares), shifting to 24°C (red circles), and shifting to 20°C (blue triangles). Arrows indicated the timing of temperature shift. Error bars represent SD of biological triplicates. (C) Effect of fermentation temperatures (30°C, 30–24°C, 30–20°C) for zeaxanthin (blue line) and crocetin (red bars) biosynthesis. (D) Crocetin and zeaxanthin production of TL009 in the media with various ratios of glucose and galactose. Error bars represent SD of biological triplicates.