Figure 2.

TSPO deficiency inhibits IL-4–induced microglial activation. WT and TSPO-knockout microglial cells were stimulated with IL-4 (1 µg/ml) for the indicated amounts of time. (A, B) RT-qPCR determined mRNA changes in the M2 markers Arg1 and CD206. The same conditions were applied to shvector and shTSPO BV2 cell lines. (C, D) Western blot analysis of Arg1 protein level changes. (E, F) RT-qPCR analysis of mRNA changes in Arg1 and CD206. Data are expressed as the mean ± SEM. Data were analyzed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.