Figure 4.

TSPO knockout dampens microglial mitochondrial function. (A, B) The mitochondrial membrane potential (Ψm) of WT and TSPO−/− microglial cells was measured by TMRM fluorescence (n=3 biological replicates). (C, D) The mitochondrial membrane potential (Ψm) of shvector and shTSPO cells was measured by TMRM fluorescence. (E) shvector and shTSPO knockdown cell lines were placed in 96-well plates at the same density, and OD450 values were detected by adding CCK at specific times. (F) The relative total mtDNA production and the amount of mtDNA released in the cytosol of WT and TSPO−/− microglia cells were quantified by RT-qPCR with primers specific for a region of mtDNA that is not inserted into nuclear DNA (non-NUMT) and primers specific for nDNA (B2m). (G) Total ATP production of WT and TSPO−/− microglial cells. Data were analyzed using one-way ANOVA. **p < 0.01, ***p < 0.001.