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. 2020 Jun 30;11:1473. doi: 10.3389/fmicb.2020.01473

Figure 4.

Figure 4

Ad5-G-Gn immunization promotes the recruitment and/or activation of dendritic cells (DCs). Groups of BALB/c mice (6–8 weeks old, female, n = 15) were immunized i.m. with 108 GFU of Ad5-G-Gn, Ad5-GFP, or DMEM (Mock). At 3, 6, and 9 dpi, single cell suspensions of inguinal lymph nodes (LNs) were prepared and stained with antibodies against DCs activation markers and analyzed via flow cytometry. The gating strategies for analyzing DCs (A) and a representative flow cytometric plot for measuring activated DCs (B). Analyses for activated DCs (C) (CD11c+ CD86+, CD11c+ MHCI+, and CD11c+ MHCII+) from the draining LNs at 3, 6, and 9 dpi are presented (*p < 0.05; **p < 0.01; ***p < 0.001).