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. 2020 Jun 30;11:1473. doi: 10.3389/fmicb.2020.01473

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Copyright © 2020 Zhao, Zheng, Yan, Sun, Xu, Zhu, Liu, Tian, He, Wei and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ad5-G-Gn immunization promotes the activation of B cells. BALB/c mice were immunized with 10⁸ GFU of Ad5-G-Gn, Ad5-GFP, or DMEM (Mock) by the i.m. route. At 3, 6, and 9 days post immunization, single cell suspensions of inguinal LNs were prepared and stained with antibodies against B cell activation markers and analyzed via flow cytometry. The gating strategies for analyzing B cells (A) and a representative flow cytometric plot for measuring activated B cells (B). Analyses for activated B cells (C) (CD19⁺ and CD40⁺) from the draining LNs at 3, 6, and 9 dpi are presented (^*p < 0.05; ^***p < 0.001).