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. 2020 Jun 30;11:1473. doi: 10.3389/fmicb.2020.01473

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Copyright © 2020 Zhao, Zheng, Yan, Sun, Xu, Zhu, Liu, Tian, He, Wei and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ad5-G-Gn immunization elicits Th1-/Th2-mediated immune responses. Enzyme-linked immunospot (ELISpot) analysis of IFN-γ and IL-4 in lymphocytes from spleens of mice immunized with Ad5-G-Gn, Ad5-GFP, or DMEM (mock) at 4 weeks after immunization. (A) RABV specific IFN-γ spot-forming cells (SFCs). (B) RABV specific IL-4 SFCs. (C) SFTSV specific IFN-γ SFCs. (D) SFTSV specific IL-4 SFCs. The representative specific SFCs images were shown below each graph. IgG isotype analysis was performed at 4 weeks after immunization and serum samples were collected and subjected to ELISA analysis of IgG2c and IgG1 antibody specific to RABV G (E) and SFTSV Gn (F). IgG titers are expressed as the reciprocals of the highest dilution of serum having a mean optical density at 405 nm greater than 2.1 times of similarly diluted negative serum samples (^*p < 0.05; ^**p < 0.01; ^***p < 0.001).