Figure 5.

Involvement of ROS in the activation of the Keap1-Nrf2 pathway. (A) Cellular H₂O₂ accumulation in HepG2 cells after treatment with 10 and 20 µM lapatinib (Lap) with or without N-acetyl cysteine (NAC, 2 mM) for 24 h. (B) Representative western blot and quantification of (C) Nrf2 and (D) Keap1 protein expression in HepG2 cells after treatment with 10 and 20 µM lapatinib (Lap) with or without N-acetyl cysteine (NAC, 2 mM) for 24 h. Data are shown as fold increase relative to the negative control (0.1% DMSO, Ctrl), and are the mean ± SEM of at least three independent replicates. ^*p < 0.05 versus negative control, ^#p < 0.05 versus the same concentration of lapatinib of the other experimental group.