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. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Nat Chem Biol. 2020 Mar 9;16(6):653–659. doi: 10.1038/s41589-020-0480-6

Fig. 4 |. Mutations that disrupt toxicity perturb formation of the amphiphilic helix.

Fig. 4 |

Membrane binding of α-synuclein point mutants measured by CD titration of purified α-synuclein with lipid vesicles ([α-synuclein] = 350 nM) or fluorescence microscopy of α-synuclein variants expressed in yeast. 2 μm scale bars are shown. Data are shown for variants with substitutions at (a) G31, (b) T33, (c) E35, or (d) V37. Experiments were performed three independent times with similar results, and representative examples are shown.