TABLE 3.

Comparison of fitness parameters for resistant Botrytis cinerea mutants and their wild-type parental isolate^a.

		In vitro		In vivo
Isolateb	Mycelial growth (cm/day)c	Sporulation (×105/cm2)	Germination rate (%)	Sporulation (×105/cm2)	Germination rate (%)	Sclerotia production (g/Petri dish)	Lesion area (mm2)
S11	2.1 ± 0.1	5.3 ± 0.7	85.4 ± 2.1	9.7 ± 1.1	96.3 ± 1.7	0.045 ± 0.006	2.9 ± 0.3
RU11-3	1.8 ± 0.2*	1.2 ± 0.1***	38.7 ± 3.4***	7.3 ± 0.3***	48.4 ± 2.1***	0.135 ± 0.012***	1.7 ± 0.3***
RU11-5	2.1 ± 0.1	0.7 ± 0.1***	0.7 ± 0.3***	5.5 ± 0.3***	61.0 ± 1.7***	0.176 ± 0.003***	2.2 ± 0.2***
RU11-7	2.0 ± 0.1	0.8 ± 0.4***	3.3 ± 0.6***	0.4 ± 0.1***	8.2 ± 2.4***	0.140 ± 0.006***	1.9 ± 0.2***
RU11-12	2.1 ± 0.1	0.8 ± 0.2***	20.5 ± 2.4***	4.9 ± 0.7***	33.3 ± 1.0***	0.173 ± 0.008***	2.2 ± 0.2***
RU11-15	2.0 ± 0.1	1.5 ± 0.2***	57.1 ± 1.6***	8.7 ± 0.3**	57.2 ± 2.6***	0.155 ± 0.024***	2.0 ± 0.2***
RU11-21	1.8 ± 0.2**	5.2 ± 0.8	34.3 ± 3.2***	9.2 ± 0.3	23.8 ± 3.3***	0.078 ± 0.007**	1.6 ± 0.1***
NJ10	2.1 ± 0.1	5.2 ± 0.7	54.2 ± 5.9	8.6 ± 0.9	90.6 ± 3.0	0.257 ± 0.032	2.2 ± 0.4
RS10-1	2.1 ± 0.2	5.2 ± 0.2	78.3 ± 4.1**	8.3 ± 0.3	92.4 ± 2.1	0.213 ± 0.029	0.3 ± 0.1***
RS10-2	2.1 ± 0.1	1.2 ± 0.4***	60.6 ± 1.2	2.5 ± 0.6***	88.5 ± 1.7	0.201 ± 0.022*	0.7 ± 0.3***
RS10-3	2.0 ± 0.1	2.3 ± 0.7***	45.8 ± 9.3	0.9 ± 1.0***	90.2 ± 2.4	0.196 ± 0.019*	1.1 ± 0.2***
RS10-4	2.1 ± 0.1	2.0 ± 0.2***	70.1 ± 3.7**	3.6 ± 0.9***	93.4 ± 1.8	0.002 ± 0.001***	1.4 ± 0.2***
RS10-5	2.0 ± 0.2	0.9 ± 0.2***	72.0 ± 6.0**	2.7 ± 1.3***	87.2 ± 5.6	0.113 ± 0.021***	1.6 ± 0.3***

^aValues in a column indicate means ± standard deviation (SD). An asterisk indicates significant differences based on an unpaired Student’s t-test with the P-values marked (^∗P < 0.05; ^∗∗P < 0.01, ^∗∗∗P < 0.001). ^bS11 and NJ10 correspond to the wild-type parental isolates. ^cGrowth rate was determined at the optimum temperature of 20°C.