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. 2020 Jul 7;6(7):e04381. doi: 10.1016/j.heliyon.2020.e04381

Table 1.

Primer sequences used for the screening and detection of the virus, the expected fragment sizes, and the references used.

Primer Target Gene Sequence (5′-3′) Fragment (bp) Reference
PanCo11Fw nsp12 TGATGATGSNGTTGTNTGYTAYAA 179 (Escutenaire et al., 2007)
PanCo13Rv GCATWGTRTGYTGNGARCARAATTC
CCV1| M TCCAGATATGTAATGTTCGG 409 (Pratelli et al., 1999)
CCV2 TCTGTTGAGTAATCACCAGCT
CENP1 N CTCGTGGYCGGAAGAATAAT 279 (Erles and Brownlie, 2009)
CENP2 GCAACCCAGAMRACTCCATC
EL1F S I CAAGTTGACCGTCTTATTATTACTGGTAG 346 (Pratelli et al., 2004)
EL1R TCATATACGTACCATTATAGCTGAAGA
S5 S IIb TGCATTTGTGTCTCAGACTT 694 (Pratelli et al., 2004)
S6 CCAAGGCCATTTTACATAAG
NSP3B-F NSP3B CTTGGTCTCTCTATTGTTGAAG 200 (De Barros et al., 2018)
NSP3B-R GCGTTGCGTTTAGAATGG
CPV PPV F VP1 GACTTGTGCCTCCAGGTTAT 420 (Demeter et al., 2009)
CPV PPV R GTTGAACTGCTCCATCACTC
GAPDH F GAPDH GCCGTGGAATTTGCCGT 164 DNA (Leutenegger et al., 1999)
GAPDH R GCCATCAATGACCCCTTCAT 82 RNA