Filaments that only partially resemble NETs, exhibiting several orders of branching, but otherwise not forming a porous matrix (A). More advanced states of NET decomposition, in which DNA appears to exhibit a much more relaxed conformation (B). NETs treated with thermally inactivated trypsin (C). In partially digested NETs, the average branch length was 278 ± 189 (s.d.) nm, indicating that proteins within NETs participate in regulating branch-length and architecture. From Pires RH. Nanoscale 2016; 8: 14193-14202. With permission.