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. 2020 Jun 30;11:1355. doi: 10.3389/fmicb.2020.01355

FIGURE 4.

FIGURE 4

Maximum likelihood phylogenetic comparison of 35 STECs from different ecosystems on the Nijkerk cattle farm and 15 E. coli/Escherichia fergusonii strains. The tree was rooted using E. fergusonii (ATCC35469) as outgroup. Phylogenetic relationships between E. coli strains were inferred by making use of 10 concatenated household genes (adk, fumC, glpK, gyrB, icd, lysA, mdh, metG, purA, and recA) with 1,000 bootstrap iterations. Relevant phylogenetic groups (A, B1, B2, D, E, and F) are indicated in the tree. Reference strain abbreviations: HS, O9:H4 strain HS; K12, O16:H48 K12 strain MG1655; O104, O104:H4 strain 2011C-3493; 55989, O104:H4 strain 55989; O103, O103:H2 strain 12009; SE11, O173:H4 strain SE11; O26, O26:H11 strain 11368; O111, O111:H8 strain 11128; IAI1, O8:H9 strain IAI1; SE15, O150:H5 strain SE15; UT189, O18:H7 strain UT189; UMNO26, O17:H18 strain UMN026; O157, O157:H7 Sakai strain; IAI39, O7:H45 strain IAI39; E. fergusonii, E. fergusonii ATCC35469.